Immunoglobulin 48G7 Germline Fab Antibody Complexed With Hapten 5-(Para-Nitrophenyl Phosphonate)-Pentanoic Acid, The Affinity Maturation Of An Esterolytic Antibody

Created by Katerina Cronin and Jon Freeman

    The protein on the right depicts one arm of the antibody.  Included are the variable light and heavy chains and the constant light and constant 1 heavy chain.  This can be seen in the circled portion of the picture posted above. 

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Original Settings

Light Chain as Cartoon

Heavy Chain as Cartoon

Cartoon

Displays heavy chain in yellow and light chain in red.

Disulfide Bridges, found between two cystein amino acids, link the light chain with the heavy chain.  Blue is the Heavy chain and Purple is the Light Chain.

Disulfide Bridge

Enlarge Disulfide Bonds (yellow)

Zoom on Disulfide Bridge (Click Original Settings TWICE Before Zooming!)

    The disulfide bridge that is zoomed on is the connecting bridge between the constant 1 heavy chain and constant light chain shown in the above diagram.

A hapten is a small molecule which can bind to an antibody but is incapable of inducing an immunogenic response unless the hapten is attached to a carrier protein.

Hapten with Cartoon Heavy & Light Chains

Electrostatic Surface:

    Add Hapten

    Remove Hapten: Note the polar region where the Hapten is bound, the red indicates that the site of attachment is electron rich.  This region is the antigen binding site which explains its polar characteristics.

The following buttons apply to the second chime image (scroll down in right window) which depicts the protein amino acids that bind to the hapten.

Original Ligand Bind Site Settings

Binding between Amino Acids and Hapten

Hapten Ball & Stick / Binding Amino Acids Tubes