Egg-White Avidin in its functional complex with Biotin Avidin is a glycoprotein made of four identical subunits. Biotin is a vitamin that is involved in carboxylation-decarboxylation reactions and forms a highly stable complex with Avidin. Two Avidin chains are contained in an asymmetric unit. Avidin's binding with biotin is one of the tightest binding intereactions known.