Procedure for Retrieving GC-MS Data from the Network
Accessing SaturnView and loading your data
After you have logged into the Windows 95, 98 or NT environment
1) a) click on Start and go to
b) Network programs
c) then to Public Access Software
d) then to Classroom Specific Software
e)and click on SaturnView
Troubleshooting with your own PC:
If you cannot access Saturn view or get the message "The file or folder 'satvw32.exe' that this shortcut refers to cannot be found," then go the My Computer and check for "Paccess on Sabre (G:). If nothing appears as drive G: and you have Windows95 or Windows98, log off and log on as studentpc with password studentpc. After logging back on as yourself, you may then have the proper G: drive, which is "Paccess on Sabre". If so, got to step 2. Otherwise check the next paragraph.
If you have another device such as a CD-Rom or share connected as drive G:, or you have WindowsNT or you tried the above, THEN call the helpdesk at 2228 for a consultation. (Information such as your username, computer network name--b_....--, operating system--95,98 or NT, what you currently have for drive G:, and your phone number will expedite the process.)
2) The last step will automatically bring you to Select Overlay Plots where you will load your data files.
a) At the top of the page click on the arrow adjacent to SaturnView
and then on organic$ on ‘lexington’(O:)
b) double-click on folder for your lab period and
c) double-click on your filename, followed by a single click on open file(s) at bottom.
This will give you the GC trace with peaks of interest in the analysis. Remember, each of these GC peaks has a mass spectrum associated with it.
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3) a) To print a copy of the GC trace, right click on the trace with the mouse and choose Print — this will give you a Chromatogram Plot — under File you can choose Print Page.
To return to the GC trace, simply click on the upper right hand corner
b) Under Spectra click on Spectrum 1, and use the mouse to point and click at the highest point of a peak of interest — a small triangle will appear on the peak and the mass spectrum will appear along side. Use the arrow keys to make sure the triangular cursor is at the top of the peak.
c) Under Spectra, click on Spectrum 2, and use mouse to point and click at the highest point of peak of interest -- again use arrow keys to make sure the cursor is at the top of the peak. Note that there is also a difference spectrum, which is irrelevant for your purposes. To remove it, right click in the difference spectrum and hide it.
d) To print a copy of the mass spectra, right click on one of the mass spectra and click on Print Spectra, followed by the process in (a). If there are other peaks of interest, you can process them in an analogous manner.
e) To obtain integration results click on Quantitation, then down to Review Results of Active File. You can copy the integration numbers on the GC trace.
To do a library search
In order to search, you must have a mass spectrum on the screen.
a) Under Search click on Library Search, then on Saturn Search where you can choose
Reverse Fit, Fit, or Purity, and the search by that routine. You can print the mass spectrum along with your choice of Hits(spectra that closely match yours).
Exiting
5) Exit by clicking on the SatView icon and choosing Close.